How to dissolve or preserve the synthesized peptide?

When possible, polypeptides should be stored as lyophilized powder at -20 ° C as much as possible to avoid bacterial degradation, secondary structure formation, oxidation, or other modifications within a few years.

The synthetic peptides provided by Simex are delivered in a lyophilized state. Generally, the dissolution of a peptide is related to the sequence of the peptide. First, try distilled water and ultrasound (1-10MG / ML). If it is insoluble, calculate the basic residues (Lys, Arg, His, and free amino terminus) and acidic residues in the peptide ( Gly, Asp and free carboxy terminus). If there are too many basic groups, you can add 1N acetic acid dropwise until it is dissolved. If there are many acidic groups, add 1N ammonia water until it is dissolved. If you need to use buffer in the experiment, it is recommended not to add salt before the peptide is completely dissolved, because the salt will reduce the solubility of the peptide. If the peptides in the above solution are not soluble, you can try using a small amount of organic solvents such as DMSO, ethyl chloride or DMF.

Cali-Bio has the most advanced technology and process in the process of peptide synthesis. It has successfully synthesized tens of thousands of high-purity peptides for many scientific research institutions, colleges and biological companies in the United States, which are used in antibody preparation and drug development. And the development of peptide vaccines and other fields. Cimejet introduced Cali-Bio's peptide synthesis service to China, and combined with China's national conditions, it aims to provide Chinese customers with special services for the quality of peptide synthesis abroad and enjoy domestic synthesis prices. For details, please click: Peptide Synthesis-Cali-Bio Peptide Synthesis Service.

For details, please click: Common Questions and Answers in Peptide Synthesis Peptide Synthesis

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