How to use the microscope, and the steps

The use of the double mirror is different, here we introduce their respective methods of use:

Method / step

1, the use of high power mirror

(1) Selecting the target: It is necessary to first adjust the part to be further observed under the low power microscope to the center, and adjust the object image to the clearest level in order to observe the high power microscope.

(2) Rotate the converter to change the high-power lens. When converting the high-power lens, the rotation speed should be slow and observe from the side (prevent the high-power lens from colliding the slide). If the high-power lens hits the slide, the focal length of the low-power lens is not Adjust it, it should be re-operated.

(3) Adjusting the focal length: After converting the high power microscope, observe it with the left eye on the eyepiece. At this time, you can usually see a less clear object, and the spiral of the fine adjuster can be moved counterclockwise by about 0.5-1 turn, ie Get a clear image (do not use a coarse regulator!)

If the brightness of the field of view is not suitable, adjust it with the concentrator and aperture. If you need to change the slide specimen, you must turn the coarse adjuster clockwise (do not turn the wrong direction) to lower the stage to remove the slide specimen.

2, low magnification mirror use

(1) Take the mirror and place it: The microscope is a high-precision instrument, so be careful when taking the mirror. Usually use the microscope, it should be stored in the cabinet or box, now go to take out, hold the arm with your right hand, hold the mirror holder with your left hand, and place the microscope firmly on the test bench in front of your left shoulder. The end of the table is 1-2 inches, which is convenient for sitting and operating.

(2) Opposite light: Move the rotator with the thumb and middle finger (do not move the lens of the hand-held object), so that the low-power lens is aligned with the light-passing hole of the stage (when the rotation hears the click, the optical axis of the objective lens is aligned with the lens barrel) center). Open the aperture, raise the concentrator, and turn the mirror to the light source, observe it on the eyepiece with the left eye (open the right eye), and adjust the direction of the mirror until the light in the field of view is even and bright.

(3) Place the slide specimen: Take a specimen of the slide on the stage, make sure that the side of the cover slip is facing up, do not reverse it, clamp it with the spring clip of the pusher, and then rotate the spinner of the pusher. , adjust the part to be observed to the center of the light hole.

(4) Adjusting the focal length: Turn the coarse adjuster counterclockwise with your left hand, so that the stage slowly rises to about 5 mm away from the specimen. Note that when looking up the stage, do not observe it on the eyepiece. Be sure to look at the stage from the right side to avoid excessive rise, resulting in damage to the lens or specimen. Then, both eyes are opened at the same time, and the left eye is observed on the eyepiece, and the left hand slowly rotates the coarse adjuster clockwise, so that the stage is slowly lowered until a clear object appears in the field of view.

If the object is not in the center of the field of view, adjust the pusher to adjust it to the center (note that the direction of the moving slide is opposite to the direction in which the object is moving). If the brightness in the field of view is not suitable, it can be adjusted by the position of the lifting concentrator or the size of the opening and closing aperture. If the stage is lowered by more than the working distance (>5.40mm) when the focal length is adjusted, the object is not seen. If the operation fails, you should re-operate, and you must not blindly and blindly raise the stage.

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