Chen Hang Company's Test Report on the Detection of Soy Lecithin with SofTA Evaporative Light Scattering Detector
Chen Hang Company used SofTA (Sofoda) evaporative light scattering detector to detect soybean phospholipid test report I. Experimental conditions Cometro (US Connaught) high performance liquid chromatography gradient system; SofTA (Sofoda) Model 300S evaporative light Scattering detector, DT = 70 ℃, SC = 30 ℃; Cometro (Connor, USA) 6000 LDI Pump; Scienhome (Tianjin Chenhang) Kromasil Sil 250 * 4.6mm column mobile phase: mobile phase A: methanol-water-ice Acetic acid-triethylamine = 85: 15: 0.45: 0.05 Mobile phase B: n-hexane-isopropanol-mobile phase A = 20: 48: 32; gradient elution time (min) mobile phase A (%) mobile phase B (%) 0 10 9020 30 7035 95 536 10 9041 10 90 Flow rate: 1ml / min; Sample: chloroform-methanol (2: 1) dissolved; Injection volume: 10ul. 2. Samples of experimental results: Channel A Results Retention Time Area Area Percent Height Height Percent 2.835 3131354 6.874 292272 13.8963.056 1875580 4.118 169116 8.0414.183 12199449 26.782 976057 46.4085.756 213382 0.468 11140 0.53022.117 27849358 61.140 647392 30.78131.248 281299 0.618 7234 0.344 Blank solvent blank gradient III. Results and discussion 1. The blank gradient shows that the baseline noise is less than 0.5mV and the drift is less than 2mV / hour. 2. The blank solvent shows that there is a solvent peak at 3 minutes. 3. The sample spectrum shows that the retention time of the main component in the sample should be 22.117min. Since there is no standard product, it is estimated to be phosphatidylcholine according to the relevant literature. Inositol, the resolution of each component in the sample meets the requirements of the Pharmacopoeia. Conclusion: Using SofTA (Sofoda) Model 300S evaporative light scattering detector to analyze soybean phospholipids, the signal-to-noise ratio is high, the baseline drift is small, and the peak out condition is good, which can meet the requirements of pharmacopoeia.
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