Leica DM IL LED Inverted Biomicroscope Operating Procedure

First, the use of the microscope due to the small size of the microorganisms, far below the observation limit of the naked eye. The size is often described in microns (um) or nanometers (nm). Therefore, to observe them, it is necessary to enlarge the system by means of a microscope. Microscopes can magnify tiny objects or tiny parts of objects.

Second, the operation steps of the microscope

1. Remove the dust cover, plug in the power socket (the power socket is usually not pulled out), and turn on the power switch.

2. Place the specimen to be inspected on the stage (check whether the objective is too high before placing it, and make sure it is lower than the center of the hole of the stage gasket).

3. Use the 10× objective lens to focus, select the corresponding filter according to the objective lens of different multiples, and adjust the spacing of the binoculars according to the eye distance of the user. 4. Adjust the microscope light to the appropriate intensity, then adjust the coarse adjustment knob to see the clear image, then use the fine adjustment knob to adjust the specimen image to the clearest. 5, after observing the overall condition of the specimen under a low power microscope, you can use a high power microscope to further observe the local condition of the specimen in detail, generally using a 40× objective lens. (Note: Before converting the objective lens, first rotate the coarse adjustment knob to lower the objective lens down slightly, and then convert between the high magnification and low magnification objective lens. This is to prevent the objective lens from hitting the lower edge of the stage to damage the objective lens.)

6. After the observation, first adjust the microscope light to the darkest, then turn off the power of the microscope. Finally, cover the microscope with a dust cover.

Third, the use of Leica Application suite V3 software

1. Turn on the computer and run the Leica Application suite V3 software.

2. After observing the required clear image, pull out the movable rod (the image can not be seen in the eyepiece, and the image can only be seen in the computer monitor), so that the light path passes through the CCD (Charge Coupled Element) to the display.

3. Click on the “Get” menu in the menu bar of the Leica Application suite V3 software to view the image.

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