Composition of elisa kit
Shanghai Yiji specializes in supplying ELISA kits (species: human; rat; mouse; guinea pig; nude mouse; hamster; chicken; pig; dog; rabbit; monkey; cow; plant; fish; duck; pea; lentil; Snake venom; goat; sheep; poultry; cat; wheat germ; horse; carp; Jing bean; aflatoxin; peanut; salmon; soybean; soybean; kidney bean; castor), teaching kit. Note: This kit is for research use only, not for clinical use !!
1. Serum: Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. After collecting blood, centrifuge at 1000 × g for 10 minutes to quickly and carefully separate the red blood cells.
2. Plasma: EDTA, citrate and heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles.
3. Cell supernatant: centrifuge at 1000 × g for 10 minutes to remove particles and polymer.
4. Tissue homogenate: Add tissue to normal saline and mash. Centrifuge at 1000 × g for 10 minutes, and take the supernatant.
5. Storage: If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. If possible, do not use hemolysis or hyperlipidemia. If there are a large number of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is thawed evenly and adequately.
This IBL kit can be used for quantitative detection of interleukin-6 in mouse serum, EDTA plasma, and cell supernatant
Kit components
1 Pre-coated plate: anti-mouse interleukin-6 rabbit IgG, affinity purified 96T
2 Enzyme labeled antibody: (30 times concentrated) HRP labeled anti-mouse interleukin-6 rabbit IgG, affinity purified 0.4mL x 1
3 Standard: Recombinant Mouse Interleukin-6 0.5mL x 2
4 EIA buffer: 1% BSA, 0.05% Tween 20 BPS 30mL x 1
5 Labeled antibody dilution: 1% BSA, 0.05% Tween 20 BPS 12mL x 1
6 Developer: TMB substrate solution 15mL x 1
7 Stop solution: 1N sulfuric acid 12mL x 1
8 Concentrated washing solution: (40 times concentrated) Contains 1% BSA, 0.05% Tween 20 BPS 50mL x 1 Operating instructions 1 Equipment needed for the experiment (but not provided by the kit) Microplate reader (450nm) Micropipette and its Nozzle cylinder and beaker Deionized water refrigerator (4 ° C) Coordinate paper (log / log) Absorbent paper test tube (for standard dilution) Incubator (37 ° C ± 1 ° C) Wash bottle (for plate washing) once Sex reagent tube (for concentrated enzyme-labeled antibody and developer)
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