Human Polysaccharide Binding Protein (LBP) ELISA Kit Usage

Detection range: 96T20pg/ml-480pg/ml Use purpose: This kit is used to determine the content of polysaccharide-binding protein (LBP) in human serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of human polysaccharide binding protein (LBP) in the specimen. The microporous plate was coated with purified human polysaccharide-binding protein (LBP) antibody to prepare a solid phase antibody, and polysaccharide binding protein (LBP) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled polysaccharide-binding protein (LBP). The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the polysaccharide binding protein (LBP) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human polysaccharide binding protein (LBP) in the sample was calculated from a standard curve. Kit composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 6ml × 1 bottle 2 Enzyme standard reagent 6ml × 1 bottle 8 standard product (960pg / ml) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes × 8 9 standard dilutions 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 color reagent A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 Bottle 12 sealed bag 1 specimen requirement 1. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided. Samples containing NaN3 could not be detected because NaN3 inhibited horseradish peroxidase (HRP) activity. Procedure 1. Dilution of the standard: This kit provides one original standard, which can be diluted in a small tube according to the following chart. 480pg/ml No.5 standard 150μl original standard added 150μl standard dilution 240pg/ml No.4 standard 150μl No.5 standard added 150μl standard dilution 120pg/ml No.3 standard 150μl No.4 standard Add 150μl standard dilution 60pg/ml No. 2 standard 150μl No. 3 standard Add 150μl standard dilution 30pg/ml No. 1 standard 150μl No. 2 standard Add 150μl standard dilution 2. Add sample: Blank holes are respectively set (the blank control hole is not added with the sample and the enzyme standard reagent, and the other steps are the same), the standard hole, and the sample hole to be tested. Accurately load 50 μl of the standard on the enzyme-labeled plate, add 40 μl of the sample dilution to the well to be tested, and then add 10 μl of the sample to be tested (the final dilution of the sample is 5 times). Add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, and shake gently to mix. 3. Incubation: The plate was sealed with a sealing film and incubated at 37 ° C for 30 minutes. 4. Solution: Dilute 30 times concentrated washing solution with distilled water 30 times and reserve. 5. Wash: carefully remove the sealing film, discard the liquid, dry it, fill each well with washing liquid, let stand for 30 seconds and discard it. , repeat this 5 times, pat dry. 6. Add enzyme: Add 50 μl of enzyme labeling reagent to each well, except for blank wells. 7. Incubation: operation is the same as 3. 8. Washing: operation is the same as 5. 9. Color development: add 50 μl of color developer A, add 50 μl of color developer B, gently shake and mix, and avoid light at 37 °C. 15 minutes. 10. Termination: 50 μl of stop solution was added to each well to terminate the reaction (when the blue color turned yellow). 11. Measurement: The absorbance (OD value) of each well was measured sequentially with a blank air conditioner of zero and a wavelength of 450 nm. The measurement should be carried out within 15 minutes after the addition of the stop solution.

Side Table

Side table is a small, versatile piece of furniture that is typically used to complement and enhance the functionality of a seating area. It is designed to be placed alongside sofas, armchairs, or beds to provide a convenient surface for placing items within easy reach. Here are some key features and aspects of a side table:

  1. Size and Shape: Side tables come in various sizes and shapes to fit different spaces and purposes. They can be round, square, rectangular, or even irregular in shape. The size of the side table should be proportional to the surrounding furniture and the available space.

  2. Materials: Side tables are made from a wide range of materials, including wood, metal, glass, and acrylic. The choice of material can impact the overall style and durability of the table. Consider the existing decor and the desired aesthetic when selecting the material for the side table.

  3. Design and Style: Side tables are available in numerous designs and styles to match different interior themes. From traditional and rustic to modern and contemporary, there are various options available. The design may feature decorative detailing, unique patterns, or sleek lines to complement the overall decor of the room.

  4. Functionality: Side tables serve a practical purpose by providing a surface for placing items within easy reach. They can be used to hold drinks, snacks, books, magazines, remote controls, or decorative items like lamps or vases. Some side tables may have additional features like drawers or shelves for extra storage.

  5. Versatility: Side tables are versatile and can be used in different areas of the home. They are commonly placed in living rooms, bedrooms, or home offices. In the living room, they can be positioned next to sofas or armchairs as a convenient spot for holding beverages or displaying decorative items. In the bedroom, they can serve as Nightstands, providing a surface for a lamp, alarm clock, or personal items.

  6. Mobility: Some side tables are designed to be lightweight and portable, making them easy to move around as needed. This can be particularly useful when rearranging furniture or when additional surface space is required for different activities.

  7. Storage Options: Depending on the design, some side tables may offer storage options such as drawers or shelves. These storage features can help keep the space organized by providing a place to store small items or display decorative pieces.

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