Shanghai Yili Biotechnology informs the use of the medium
The medium is placed in a boiling water bath or melted by a method that has the same effect (such as laminar steam in an autoclave). The high pressure medium should minimize the reheating time and avoid excessive heating after melting.
After melting, it should be placed at room temperature for a short time (such as 2 min) to avoid broken glass bottles. The melted medium is placed in a constant temperature water bath at 47 ° C to 50 ° C to cool and keep warm (the water bath temperature can be appropriately increased according to the actual medium solidification temperature) until use. The thawed medium should be used as soon as possible, and the storage time should not exceed 4 h.
Unused media cannot be re-coagulated for later use. In particular, sensitive media should be noted that the incubation time should be as short as possible after melting. If there are specific requirements, refer to the specified standards. The temperature of the medium poured into the sample should be controlled to about 45 °C.
The heat-labile additive should be added when the medium is cooled to 47 ° C to 50 ° C. Sterile ingredients should be placed at room temperature prior to addition to prevent the agar from coagulating or forming a sheet. The medium to which the added component is added is slowly and thoroughly mixed, and dispensed into a container to be used as soon as possible.
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