How to choose the light source of fluorescence microscope?
When we choose the light source of the fluorescence microscope (Fluorescence Microscope), we should pay attention to the size of the arc, that is, the size of the light emitting area of ​​the light source, such as a 50W mercury lamp, its arc size is 110X0.13MM, 200W arc size is 212X0. 15MM, and the arc size of 100W is 0.125 × 0.125m, whether it can just fill the entrance pupil of the system so that the light can be fully utilized, an excellent fluorescence microscope (Fluorescence Microscope), and various magnification objectives "light energy" Can be fully and effectively used is a worthy indicator. In the design of fluorescence microscopes, the choice of light source is crucial in the overall design. It determines the scope and cost of the instrument and also reflects the grade of the instrument. Microscopes above the laboratory should use mercury lamps as the main light source, and simple (special) microscopes usually use halogen lamps as the main light source.
Secondly, the question we still need to consider is its power. Do we use high-power bulbs or choose low-wattage bulbs? In principle, low-wattage light sources should be selected as much as possible, which is undoubtedly beneficial to structural design and cost of. However, when the wattage is too low, the fluorescence cannot be excited or the fluorescence emitted is unstable, so it cannot be used. However, whether it can excite fluorescence is related to the use of light energy of the entire imaging system. How to fully and reasonably and effectively use light energy can reduce the wattage, but if we simply pursue high-power light sources as fluorescence detection, it is not desirable , Because it causes fluorescence to decay and quench. The currently available epi-fluorescence microscopes are only equipped with 100W mercury or xenon and halogen lamps.
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