With the increasing use of electronic technology in all aspects of daily life, the use of a large number of sophisticated and low-cost sensors has become a trend for future development. As can be seen from the market data, the demand for a large number of suitable and reliable sensors is increasing. However, biosensors have not been popularized because of their profound and complicated working principles and high prices.

Screen printing has been widely used in the electronics industry since the 1960s and has become an indispensable production process in the preparation of electronic devices. A patent document in 1981 reported that screen printing technology was applied to the production of thick film electrochemical sensors, which aroused great interest of many researchers. Since then, reports on screen printing biosensors have been common.

Therefore, people began to have a new concept for biosensors, that is, the birth of disposable biosensors (also known as thick film enzyme electrodes) that do not need to be reused completely solves the complex manufacturing process, high production cost, and practicality of traditional enzyme electrodes. Poor sex, inconvenient operation and other problems.

After several years of development, screen printing technology has developed into a mature key technology for the production of biosensors, and has been widely used as a technical guarantee for the market of biosensors. At the same time, high-tech products such as biosensors have come down from the mysterious "ivory tower" and walked into every hospital and every family, and they can operate their own testing instruments without professional technical training. The screen printing process has led noble biosensors to low-cost, easy-to-use, disposable throwaway detection instruments, known as "user-friendly" biosensors.

At present, the most studied and most popular glucose sensor is glucose oxidase, which is made by screen printing process, and can be printed on a single piece at a time, forming hundreds of sensors, which greatly reduces the enzyme sensor. Production costs. Relevant screen printing biosensors are involved in many books and publications, but the specific process of making enzyme electrodes by screen printing is rarely reported. Even if there are, it is also foggy and unclear, and the content lacks depth. The operability is not strong, which is not convenient for readers to learn and adopt. Here, the author makes a more detailed introduction to this, for the reference study of interested friends.

Biosensors generally have two main components: one is a biomolecule recognition element (receptor), and the other is a signal converter (transducer). For the enzyme electrode sensor, the above two components are completed on the enzyme electrode at one time, so the enzyme electrode is a biosensor composed of an immobilized enzyme and an electrochemical electrode such as an ion selective electrode, a gas sensing electrode, a redox electrode, etc. .

The process of screen printing electrode will introduce the process of screen printing electrode one by one.

1. Selection of silk screen The silk screen is the support of the silk screen printing plate, and also the base of the silk screen printing plate. At present, there are various quality, grade and width wire mesh supply on the market, and users should choose according to their needs and experience. However, there are few domestic varieties and incomplete specifications. For high-density, high-strength, low-stretch wire, anti-static, and calendering, wire mesh needs to be imported. It is better to choose imported technology products.

In recent years, in addition to the use of polyester and stainless steel wire mesh in the past, electronic components have also emerged with high-tension polyester yarn core and polyamide core sleeve. This mesh yarn is made of polypropylene fiber It is made with a mesh number of 330 mesh, a wire diameter of 23μm, interwoven into 36μm, and the mesh yarn can obtain high tension through plasma treatment, metal evaporation, polishing and other treatments, which can meet the requirements of precision. In addition, a new type of non-woven silk screen has been developed abroad, which overcomes the trouble caused by the traditional woven screen coated with photoresist and adapts to the requirements of advanced ultra-fine graphic screen printing.

In addition to the above, when selecting the appropriate number of meshes, the matching of the following parameters should also be considered:

(1) Matching of screen mesh and ink system: Ink manufacturers generally provide information on the screen that various inks should use. For example, the conductive fillers in carbon conductive inks include carbon black, graphite, carbon fiber, etc., all have stable chemical properties, and the conductive properties are worse than silver conductive inks, but their prices are cheaper. Regardless of the carbon or silver conductive ink, the particle size of the conductive filler in the ink must be considered. Take silver conductive ink as an example: the size of silver powder particles in silver conductive ink does not match the number of selected meshes, which will cause large particles to be difficult to be blocked by too fine screens during printing. Generally 180 to 240 mesh screens are used. Large, because conductive ink such as silver paste is a mixture of fine silver particles with good conductivity and conductive resin solvent. The size of silver metal particles, the solid content of silver and the shape of metal silver particles of different manufacturers are different. Silver particles are the main component that determines the electrode resistance. When printing, you should try to make the conductive silver particles smoothly pass through the screen. If you choose a mesh with a fine mesh size, although the printed electrode lines are straight and the shape is beautiful, the internal structure is loose. The content of silver particles is small (most of the silver powder fails). The electrode pattern not only has a large resistance, but also is unfavorable to the current and life, and it is difficult to satisfy the application.

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