Vitamins

Vitamin Eagle's MEM contains only water-soluble vitamins (Group B vitamins, choline, folic acid, glycol, nicotine, but does not include biotin), including serum-free medium M199 CMRL1066 (from M199) and RPMI1640 contain p- PABA. Although LHC-9 contains vitamin A and MCDB110 contains vitamin E, only M199 contains all fat-soluble vitamins (A, D, E, K), and certain vitamins in serum-free medium (e.g. (Choline and nicotine) concentration has increased. The amount of vitamins added is limited, for example, folic acid will precipitate in a concentrated storage solution, it is generally believed that this limit determines the degree of reduction in cell death to growth ratio, and It is not the maximum cell density. Like some amino acids, the vitamin requirement is based on experience, and it is usually related to the culture medium originally used during the establishment of a cell line.For example, Fisher's medium contains high concentration of folic acid, which is Because of the dependence of L5178Y on folic acid, folic acid was used in the development of this medium.

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