Working Principle of Shanghai Guning Instrument Chromatography Cabinet

The working principle of the chromatography cabinet: there are many specific interactions between biological molecules, and they can be combined specifically and reversibly. This binding force is called affinity. Affinity chromatography is the separation and purification of the other molecule by fixing one of the two molecules with affinity on an insoluble matrix, using the specificity and reversibility of the intermolecular affinity. The molecules immobilized on the matrix are called ligands. The ligands and the matrix are covalently bound to form the stationary phase of affinity chromatography, which is called affinity adsorbent. In affinity chromatography, the substance with affinity to the biological macromolecule to be separated is first selected as a ligand, and the ligand is covalently bound to a suitable insoluble matrix. The prepared affinity adsorbent is packed into a column to equilibrate. When the sample solution passes through the affinity chromatography column, the biomolecules to be separated will specifically bind to the ligand and thus remain on the stationary phase; The ligand binds and is still in the mobile phase and flows out with the eluent, so that there are only the biological molecules to be separated in the chromatography column. By eluting it from the ligand through an appropriate eluent, the purified material to be separated is obtained.